Abstract: Objective　To establish a drowning death rat model and analyze the changes of nucleotide and stress protein levels in thyroid at different time after drowning treatment.Methods　Male Wistar rats were divided into a control group and an experimental group. The control group (50 rats) was subjected to painless euthanasia by ether inhalation, while the experimental group (50 rats) was subjected to drowning in a water tank.5 rats were collected at 0, 2, 4, 6, 8, 12, 18, 24, 36 and 72 h after death, respectively. The thyroid glands were removed. 20 mg was weighed and ground respectively, and then centrifuged for 10 minutes using a low-speed refrigerated centrifuge at 3 500 r/min and the supernatant was obtained by enzyme-linked immunoassay to detect the levels of cAMP, cGMP, c-Fos, Hsp-70, Caspase-9, and Bax according to the instructions of the ELISA kit.Results　The levels of cAMP, cGMP, c-Fos, Hsp-70, Caspase-9 and Bax in thyroid tissue were higher than those in control group at different time after drowning death (P<0.05,P<0.01).The levels of cAMP, cGMP, c-Fos, Hsp-70, Caspase-9 and Bax in both the experimental group and the control group increased gradually from 2 h after death.Hsp-70 to 18 h begins to decrease,cAMP, cGMP and c-Fos began to decrease at 24 h, and Caspase-9 and Bax began to decrease at 36 h.Conclusion　The levels of nucleotide and stress protein in tissues showed a certain trend at different time after death.

Key words: drowning death, thyroid gland, nucleotide, stress protein, male rat

摘要： 目的　建立溺水死亡大鼠模型,检测分析溺水处理后不同时间甲状腺核苷酸和应激蛋白水平的变化。方法 将对照组雄性大鼠呼吸麻醉安乐死,实验组麻醉后置于水盆中溺水处理,分别在死亡后0、2、4、6、8、12、18、24、36 和 72 h各取5只大鼠,剥取甲状腺,分别称取20 mg研磨,用低速冷冻离心机3 500 r/min离心10 min,取上清液用酶 联免疫检测仪按ELISA试剂盒说明书检测环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)和原癌基因fos(c-Fos)、热休 克蛋白70(Hsp-70)、胱天蛋白酶9(Caspase-9)、Bcl-2相关X蛋白(Bax)水平。结果　雄性大鼠溺水死亡后不同时 间甲状腺组织cAMP、cGMP、c-Fos、Hsp-70、Caspase-9、Bax水平均高于对照组(P<0.05,P<0.01)。实验组和对照组 cAMP、cGMP、c-Fos、Hsp-70、Caspase-9、Bax水平均从死亡2 h后逐渐升高,Hsp-70从18 h开始降低,cAMP、cGMP、 c-Fos从24 h开始降低,Caspase-9、Bax水平从36 h开始降低。结论　动物死亡后不同时间组织核苷酸和应激蛋白 水平的变化呈现一定的趋势。

关键词: 溺水死亡, 甲状腺, 核苷酸, 应激蛋白, 雄性大鼠